6CH9
Crystal structure of a natively-glycosylated B41 SOSIP.664 HIV-1 Envelope Trimer in complex with the broadly-neutralizing antibodies BG18 and 35O22
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Envelope glycoprotein gp41 | polymer | 153 | 17357.8 | 1 | UniProt (B3UEZ6) Pfam (PF00517) | Human immunodeficiency virus 1 | Env polyprotein |
| 2 | B (D) | 35O22 Heavy Chain | polymer | 243 | 26171.5 | 1 | Homo sapiens (Human) | ||
| 3 | C (E) | 35O22 Light Chain | polymer | 216 | 23318.8 | 1 | Homo sapiens (Human) | ||
| 4 | D (G) | Envelope glycoprotein gp120 | polymer | 518 | 57929.7 | 1 | UniProt (B3UES2) Pfam (PF00516) | Human immunodeficiency virus 1 | Env polyprotein |
| 5 | E (Q) | BG18 Heavy Chain | polymer | 240 | 26020.2 | 1 | Homo sapiens (Human) | ||
| 6 | F (R) | BG18 Light Chain | polymer | 215 | 23021.4 | 1 | Homo sapiens (Human) | ||
| 7 | G, H, J, L, Q... (A, C, H, J, O...) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 7 | In PDB GlyTouCan (G15407YE) | |||
| 8 | I (F) | alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G22573RC) | |||
| 9 | K, M, P (I, K, N) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 10 | N (L) | alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G34442SS) | |||
| 11 | O (M) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G81315DD) | |||
| 12 | S (S) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 13 | U (U) | alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G00850LT) | |||
| 14 | V, W, X, Y (B, G) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 4 | Chemie (NAG) |
Sequence modifications
B: 512 - 664 (UniProt: B3UEZ6)
G: 31 - 507 (UniProt: B3UES2)
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 563 | conflict |
| Cys 605 | Thr 609 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Asp -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Lys 0 | - | expression tag |
| Arg 1 | - | expression tag |
| Gly 2 | - | expression tag |
| Leu 3 | - | expression tag |
| Cys 4 | - | expression tag |
| Cys 5 | - | expression tag |
| Val 6 | - | expression tag |
| Leu 7 | - | expression tag |
| Leu 8 | - | expression tag |
| Leu 9 | - | expression tag |
| Cys 10 | - | expression tag |
| Gly 11 | - | expression tag |
| Ala 12 | - | expression tag |
| Val 13 | - | expression tag |
| Phe 14 | - | expression tag |
| Val 15 | - | expression tag |
| Ser 16 | - | expression tag |
| Pro 17 | - | expression tag |
| Ser 18 | - | expression tag |
| Gln 19 | - | expression tag |
| Glu 20 | - | expression tag |
| Ile 21 | - | expression tag |
| His 22 | - | expression tag |
| Ala 23 | - | expression tag |
| Arg 24 | - | expression tag |
| Phe 25 | - | expression tag |
| Arg 26 | - | expression tag |
| Arg 27 | - | expression tag |
| Gly 28 | - | expression tag |
| Ala 29 | - | expression tag |
| Arg 30 | - | expression tag |
| Cys 501 | Ala 505 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 173819.6 | |
| Branched | Number of molecules | 15 |
| Total formula weight | 9933.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 884.8 | |
| All* | Total formula weight | 184637.5 |
