6CH7
XFEL crystal structure of a natively-glycosylated BG505 SOSIP.664 HIV-1 Envelope Trimer in complex with the broadly-neutralizing antibodies BG18 and 35O22
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE MFX |
| Synchrotron site | SLAC LCLS |
| Beamline | MFX |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-06 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.301 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 238.944, 238.944, 354.014 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.030 - 3.800 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.26200 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.160 |
| Data reduction software | cctbx.xfel |
| Data scaling software | cctbx.prime |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.570 | 3.870 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.535 | |
| Number of reflections | 38101 | 1759 |
| <I/σ(I)> | 4.31 | |
| Completeness [%] | 99.1 | 93.2 |
| Redundancy | 9.31 | |
| CC(1/2) | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 5% Tacismate pH 8.0, 14% PEG 3350, 0.1M Tris pH 8.0 |
