6CCB

Crystal structure of 253-11 SOSIP trimer in complex with 10-1074 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03327
Spacegroup name	H 3
Unit cell lengths	237.284, 237.284, 282.044
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.547 - 6.500
R-factor	0.3047
Rwork	0.298
R-free	0.36580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t3x
RMSD bond length	0.002
RMSD bond angle	0.539
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.600	6.740
High resolution limit [Å]	6.500	6.500
Rmerge	0.070	0.560
Rpim	0.050	0.400
Number of reflections	11600	5468
<I/σ(I)>	11.9
Completeness [%]	99.2
Redundancy	2.9
CC(1/2)	0.996	0.491

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.5 M sodium chloride, 1.9 M ammonium sulphate and 0.1 M sodium cacodylate, pH 6.5