6CBO
X-ray structure of GenB1 from micromonospora echinospora in complex with neamine and PLP (as the external aldimine)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9498 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.888, 59.980, 210.694 |
| Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.15685 |
| Rwork | 0.155 |
| R-free | 0.18818 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.735 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.048 | 0.111 |
| Number of reflections | 201237 | 18111 |
| <I/σ(I)> | 36.4 | 10.5 |
| Completeness [%] | 92.3 | 83.4 |
| Redundancy | 2.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 17-19% PEG-8000, 2% 2-propanol, 1 mM PLP, 5 mM neamine, 100 mM MES |
