6C7A
Conformational Changes in a Class A Beta lactamase that Prime it for Catalysis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Detector technology | CCD |
Collection date | 2015-01-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 71.825, 71.825, 96.534 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.270 - 1.050 |
Rwork | 0.120 |
R-free | 0.15000 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.270 | 1.110 |
High resolution limit [Å] | 1.050 | 1.050 |
Rmerge | 0.075 | 0.370 |
Rpim | 0.020 | 0.149 |
Number of reflections | 134116 | |
<I/σ(I)> | 5.8 | 2 |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 9.2 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 6.1 | 293 | 10 mg/ml protein in a solution containing 2.0 M ammonium sulfate and 0.1 M sodium citrate (pH 6.1) |