6C6Q
Crystal Structure of the Murine Norovirus VP1 P Domain in complex with the CD300lf Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2017-10-02 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.712, 135.245, 139.516 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.997 - 2.000 |
| R-factor | 0.1833 |
| Rwork | 0.182 |
| R-free | 0.22220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 3LQ6 & 5FFL |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.600 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.8.0) |
| Refinement software | PHENIX (1.12) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 62.000 | 62.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.179 | 0.058 | 1.965 |
| Rmeas | 0.195 | 0.064 | 2.214 |
| Rpim | 0.076 | 0.026 | 0.987 |
| Total number of observations | 378017 | ||
| Number of reflections | 57651 | 840 | 2644 |
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 91.2 | 99.8 | 57.8 |
| Redundancy | 6.6 | 6.2 | 4.7 |
| CC(1/2) | 0.994 | 0.996 | 0.401 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | 0.1 M Tris, pH 8.7, 0.1 M magnesium chloride, 10% PEG8000 |
