6C63
Crystal Structure of the Mango-II Fluorescent Aptamer Bound to TO1-Biotin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.495 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 36.831, 182.412, 107.494 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.206 - 2.900 |
| R-factor | 0.189598873662 |
| Rwork | 0.185 |
| R-free | 0.22955 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5v3f |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.370 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.206 | 2.990 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.058 | 0.340 |
| Number of reflections | 15464 | 1257 |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 8.8 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M HEPES pH 7.5, 2.45 M Ammonium Formate, 14.5% glycerol, 6.5% D-Sorbitol |
