6BZ8

Thermus thermophilus 70S containing 16S G347U point mutation and near-cognate ASL Leucine in A site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	210.805, 448.832, 618.447
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	134.676 - 3.740
R-factor	0.2054
Rwork	0.204
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v97
RMSD bond length	0.010
RMSD bond angle	1.107
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	134.676	134.676	3.700
High resolution limit [Å]	3.610	16.140	3.610
Rmerge	0.225	0.049	1.253
Rmeas	0.235	0.052	1.352
Total number of observations	7986683
Number of reflections	666085	7954	49062
<I/σ(I)>	9.79	40.01	1.52
Completeness [%]	100.0	99.3	99.9
Redundancy	11.99	12.664	7.036
CC(1/2)	0.991	0.989	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	4.7% PEG 20k, 4.7% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC