6BZ1

MEF2 Chimera D83V mutant/DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-02-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 32
Unit cell lengths	78.440, 78.440, 111.041
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.990 - 2.970
R-factor	0.14091
Rwork	0.139
R-free	0.18997
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.525
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.930	3.107
High resolution limit [Å]	3.000	3.000
Number of reflections	15258
<I/σ(I)>	41.66
Completeness [%]	99.7
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.1M MES pH 5.94, 0.2 M NaCl and 18% PEG2000MME