6BYX

Complex structure of LOR107 mutant (R259N) with tetrasaccharide substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-15
Detector	RAYONIX MX300HE
Wavelength(s)	0.9787
Spacegroup name	P 21 21 21
Unit cell lengths	83.050, 120.959, 127.221
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.890 - 2.208
R-factor	0.2053
Rwork	0.204
R-free	0.25460
RMSD bond length	0.008
RMSD bond angle	0.933
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13rc2_2981: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.890	2.260
High resolution limit [Å]	2.210	2.210
Number of reflections	64618
<I/σ(I)>	23.8
Completeness [%]	99.0
Redundancy	14.8
CC(1/2)	1.000	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	25.5% PEG8K, 0.17M Ammonium Sulfate, 0.1M Mes pH 6.5, 15% Glycerol