6BW4
Crystal structure of RBBP4 in complex with PRDM16 N-terminal peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.034, 59.862, 101.843 |
| Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
| Resolution | 19.870 - 2.000 |
| R-factor | 0.2004 |
| Rwork | 0.199 |
| R-free | 0.23330 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6bw3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.485 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.870 | 19.870 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.058 | 0.022 | 0.738 |
| Rmeas | 0.067 | 0.026 | 0.847 |
| Rpim | 0.033 | 0.012 | 0.411 |
| Total number of observations | 2497 | 18165 | |
| Number of reflections | 61417 | 677 | 4483 |
| <I/σ(I)> | 13.8 | 47 | 2.4 |
| Completeness [%] | 99.2 | 91.3 | 99.3 |
| Redundancy | 3.9 | 3.7 | 4.1 |
| CC(1/2) | 0.999 | 0.999 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 22% PEG3350, 0.2 M Sodium malonate, 0.1 M BisTris |
