6BVJ

Ras:SOS:Ras in complex with a small molecule activator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.98
Spacegroup name	I 4 2 2
Unit cell lengths	184.329, 184.329, 179.083
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.109 - 1.747
R-factor	0.1562
Rwork	0.155
R-free	0.16790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nyi
RMSD bond length	0.007
RMSD bond angle	0.855
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12rc1_2801: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.110	1.810
High resolution limit [Å]	1.747	1.747
Rpim	0.033	0.296
Number of reflections	153914
<I/σ(I)>	18.4
Completeness [%]	99.9
Redundancy	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4	292	0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0