6BP2
Therapeutic human monoclonal antibody MR191 bound to a marburgvirus glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 133.796, 133.796, 151.003 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.167 - 3.172 |
| R-factor | 0.2536 |
| Rwork | 0.252 |
| R-free | 0.28370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3X2D |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.651 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.170 | 3.290 |
| High resolution limit [Å] | 3.170 | 3.170 |
| Rmerge | 0.160 | 2.020 |
| Rmeas | 0.170 | |
| Number of reflections | 26795 | 2652 |
| <I/σ(I)> | 12.4 | 1.3 |
| Completeness [%] | 99.3 | 98.38 |
| Redundancy | 11 | 11.5 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 100 mM HEPES pH 7.5, 5% PEG 3000, 26% PEG 400, 6% Glycerol |
