6BP1
Crystal structure of human KRAS A59G mutant in complex with GCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979320 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.391, 47.473, 89.665 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.595 - 2.001 |
| R-factor | 0.2028 |
| Rwork | 0.202 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4obe |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_2932) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.093 | 0.315 |
| Rpim | 0.041 | 0.141 |
| Number of reflections | 10450 | 514 |
| <I/σ(I)> | 20.32 | 5.37 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.8 | 5.8 |
| CC(1/2) | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 4% MPD, 0.1 M citric acid, pH 3.5, 20% PEG1500 |
