6BOG
Crystal structure of RapA, a Swi2/Snf2 protein that recycles RNA polymerase during transcription
Replaces: 3DMQExperimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97935,0.97951 |
Spacegroup name | P 43 |
Unit cell lengths | 123.856, 123.856, 187.994 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.193 - 3.205 |
R-factor | 0.25 |
Rwork | 0.244 |
R-free | 0.27890 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.900 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.310 |
High resolution limit [Å] | 3.200 | 6.870 | 3.200 |
Rmerge | 0.123 | 0.061 | 0.966 |
Rmeas | 0.139 | 0.069 | 1.112 |
Rpim | 0.064 | 0.032 | 0.539 |
Total number of observations | 203007 | ||
Number of reflections | 45885 | 4515 | 4534 |
<I/σ(I)> | 10.5 | ||
Completeness [%] | 99.0 | 95.9 | 98.5 |
Redundancy | 4.4 | 4.5 | 3.8 |
CC(1/2) | 0.995 | 0.426 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | Ammonium sulfate, 1,4-Dioxane, Ethylene glycol, MES |