6BLI

RSV G peptide bound to Fab CB002.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.033
Spacegroup name	C 1 2 1
Unit cell lengths	113.760, 85.230, 214.300
Unit cell angles	90.00, 95.03, 90.00

Refinement procedure

Resolution	43.128 - 2.120
R-factor	0.1714
Rwork	0.169
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4npy 5it2
RMSD bond length	0.008
RMSD bond angle	0.974
Data reduction software	MOSFLM (7.2.1)
Data scaling software	Aimless (0.5.17)
Phasing software	PHASER (7.0.033)
Refinement software	PHENIX (1.11.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.130	2.160
High resolution limit [Å]	2.120	2.120
Rmerge	0.174	0.487
Number of reflections	114818	5719
<I/σ(I)>	4
Completeness [%]	99.2	99.5
Redundancy	2.9	2.8
CC(1/2)	0.988	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	14.9% (w/v) PEG 3,350 and 0.1 M succinic acid.