6BKB

Structure of Hepatitis C Virus Envelope Glycoprotein E2 core from genotype 6a bound to broadly neutralizing antibody AR3A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	60.922, 67.559, 218.353
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.382 - 2.799
R-factor	0.2316
Rwork	0.230
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mwf
RMSD bond length	0.003
RMSD bond angle	0.573
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.598	2.600
Rpim	0.030
Number of reflections	26290	998
<I/σ(I)>	19.6	1
Completeness [%]	91.7	46.2
Redundancy	10.9	2.8
CC(1/2)		0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	293	20% (w/v) PEG 3500, 0.2M sodium-citrate