6BIY
HLA-DRB1 in complex with Histone 2B peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 67.826, 182.478, 76.807 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.223 - 2.050 |
R-factor | 0.185114762639 |
Rwork | 0.183 |
R-free | 0.23185 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mdi |
RMSD bond length | 0.007 |
RMSD bond angle | 0.866 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.220 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.053 | |
Number of reflections | 30243 | |
<I/σ(I)> | 9.9 | |
Completeness [%] | 99.6 | |
Redundancy | 4.4 | |
CC(1/2) | 0.989 | 0.739 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 294 | 20-28% PEG 3350, 0.2M Potassium Nitrate, 0.1M Bis-Tris-Propane pH 7.3 |