6BI2
Trastuzumab Fab D185A (Light Chain) Mutant Biotin Conjugation.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97648 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.646, 69.139, 91.471 |
| Unit cell angles | 109.80, 100.29, 90.08 |
Refinement procedure
| Resolution | 37.370 - 1.801 |
| R-factor | 0.1675 |
| Rwork | 0.166 |
| R-free | 0.20210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n8z |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.908 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.047 | 0.461 |
| Rmeas | 0.055 | 0.538 |
| Rpim | 0.028 | 0.276 |
| Number of reflections | 78712 | 7683 |
| <I/σ(I)> | 25 | 2.14 |
| Completeness [%] | 97.5 | 95.1 |
| Redundancy | 3.9 | 3.7 |
| CC(1/2) | 0.882 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 1.0% trptone, 20% PEG 3350, 100 mM HEPES pH 7.0 |
