6BHY

Mouse Immunoglobulin G 2c Fc fragment with single GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-08-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	103.233, 89.215, 69.851
Unit cell angles	90.00, 132.20, 90.00

Refinement procedure

Resolution	44.608 - 2.040
R-factor	0.2132
Rwork	0.210
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rgs
RMSD bond length	0.019
RMSD bond angle	1.873
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.238	2.113
High resolution limit [Å]	2.040	2.040
Rmerge	0.093	0.553
Rmeas	0.109	0.655
Number of reflections	29903	2977
<I/σ(I)>	8.95	1.75
Completeness [%]	100.0	100
Redundancy	3.7	3.3
CC(1/2)	0.995	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291.15	50mM Tris pH 8 with 10% PEG 6K