6BHR

HIV-1 immature CTD-SP1 hexamer in complex with IP6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 6
Unit cell lengths	70.939, 70.939, 42.041
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.469 - 2.908
R-factor	0.2188
Rwork	0.215
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i4t
RMSD bond length	0.005
RMSD bond angle	1.090
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.900
Number of reflections	2665
<I/σ(I)>	10.3
Completeness [%]	96.3
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		290	0.2 M NaCl 20% PEG 3350 0.1 M Bis-Tris pH 5.35