6BHJ
Structure of HIV-1 Reverse Transcriptase Bound to a 38-mer Hairpin Template-Primer RNA-DNA Aptamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.055, 127.463, 132.125 |
| Unit cell angles | 90.00, 101.81, 90.00 |
Refinement procedure
| Resolution | 47.692 - 2.810 |
| R-factor | 0.2048 |
| Rwork | 0.203 |
| R-free | 0.24440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d3g |
| RMSD bond length | 0.038 |
| RMSD bond angle | 1.086 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.094 | 0.624 |
| Rmeas | 0.107 | 0.742 |
| Rpim | 0.050 | 0.391 |
| Number of reflections | 65782 | 6113 |
| <I/σ(I)> | 12.6 | 1.64 |
| Completeness [%] | 93.0 | 90 |
| Redundancy | 4.1 | 3.1 |
| CC(1/2) | 0.877 | 0.461 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 278 | 10% PEG 8000, 25 mM BisTris-Propane pH 6.8, 75 mM BisTris-Propane pH 7.4, 50 mM Ammonium Sulfate, 5% glycerol, 5% sucrose |
