6BG2
Crystal structure of cGMP-dependent protein kinase Ialpha (PKG Ialpha) catalytic domain in AMP-PNP bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2015-09-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97741 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.390, 98.500, 116.424 |
| Unit cell angles | 90.00, 133.47, 90.00 |
Refinement procedure
| Resolution | 47.231 - 1.830 |
| R-factor | 0.1884 |
| Rwork | 0.188 |
| R-free | 0.21550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fjq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.782 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.230 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 117904 | 5795 |
| <I/σ(I)> | 16.8 | |
| Completeness [%] | 99.0 | 98.3 |
| Redundancy | 4.7 | 4.8 |
| CC(1/2) | 0.999 | 0.698 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 23.2% PEG1500, 0.08 M PCTP, pH8.0, 0.5% w/v polyvinylpyrrolidone K15 |
