6BFS
The mechanism of GM-CSF inhibition by human GM-CSF auto-antibodies
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.954 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.454, 48.129, 77.602 |
Unit cell angles | 90.00, 99.86, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
R-factor | 0.185 |
Rwork | 0.182 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rjl |
RMSD bond length | 0.017 |
RMSD bond angle | 1.842 |
Data scaling software | Aimless (0.5.4) |
Phasing software | PHASER (2.5.7) |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 39.550 | 2.050 |
High resolution limit [Å] | 2.000 | 8.950 | 2.000 |
Rmerge | 0.074 | 0.031 | 0.613 |
Rmeas | 0.087 | 0.037 | 0.714 |
Rpim | 0.045 | 0.019 | 0.365 |
Number of reflections | 34696 | 421 | 2557 |
<I/σ(I)> | 12 | ||
Completeness [%] | 99.4 | 98.4 | 99 |
Redundancy | 3.7 | 3.4 | 3.8 |
CC(1/2) | 0.998 | 0.998 | 0.772 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 283.15 | 0. 2 M Sodium Chloride, 20% PEG 8000, 0.1 M phosphate Citrate 4.5 |