6BFN

Crystal structure of human IRAK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	88.059, 88.059, 177.034
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.844 - 2.260
R-factor	0.1861
Rwork	0.184
R-free	0.22690
RMSD bond length	0.005
RMSD bond angle	0.916
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.060
High resolution limit [Å]	2.260	2.260
Number of reflections	33471
<I/σ(I)>	13.3
Completeness [%]	100.0
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	20% PEG3350, 0.2 M calcium chloride, 0.1 M HEPES, pH 7.0