6BEK

Structure of sIHF bound to an 8bp palindromic DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-01-26
Detector	PSI PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	42.260, 72.110, 103.257
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.380 - 1.700
R-factor	0.2025
Rwork	0.201
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4itq
RMSD bond length	0.010
RMSD bond angle	1.163
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.064	0.366
Rpim	0.028	0.162
Number of reflections	35669	1740
<I/σ(I)>	22.6	3.9
Completeness [%]	99.7	99.8
Redundancy	6.2	6
CC(1/2)	0.977	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	277	0.1 M HEPES pH 7.6, 0.2 M KSCN, 19% (w/v) PEG 3350, and 5% (v/v) ethylene glycol