6BCA
A Complex between PH Domain of LbcRhoGEF (AKAP-Lbc) and Activated RhoA Bound to a GTP Analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.346, 60.525, 63.618 |
| Unit cell angles | 91.83, 92.97, 90.01 |
Refinement procedure
| Resolution | 34.212 - 1.995 |
| R-factor | 0.1982 |
| Rwork | 0.196 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.532 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.212 | 2.030 |
| High resolution limit [Å] | 1.995 | 1.995 |
| Number of reflections | 50558 | 2327 |
| <I/σ(I)> | 11 | 1.8 |
| Completeness [%] | 97.4 | 90.7 |
| Redundancy | 2.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 24-26% PEG 3350, 100mM Bis-Tris, 200mM ammonium sulfate |
