6BBN
Crystal structure of a curved tubulin complex induced by the kinesin-13 Kif2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 145.052, 82.188, 147.658 |
| Unit cell angles | 90.00, 109.33, 90.00 |
Refinement procedure
| Resolution | 49.217 - 3.514 |
| R-factor | 0.2391 |
| Rwork | 0.236 |
| R-free | 0.28740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 4LNU & 2GRY |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.689 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.580 |
| High resolution limit [Å] | 3.460 | 7.450 | 3.460 |
| Rmerge | 0.212 | 0.041 | 2.235 |
| Rmeas | 0.249 | 0.049 | 2.685 |
| Rpim | 0.131 | 0.025 | 1.469 |
| Total number of observations | 142449 | ||
| Number of reflections | 40041 | 4151 | 3869 |
| <I/σ(I)> | 4.5 | ||
| Completeness [%] | 99.3 | 99.7 | 96.9 |
| Redundancy | 3.6 | 3.6 | 3.1 |
| CC(1/2) | 0.998 | 0.212 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 8% PEG8000, 6% ethylene glycol, 10 mM DDT, 100 mM HEPES, pH 7.5 |
