6BBA
Crystal structure of human mitochondrial ClpP complex with acyldepsipeptide ADEP-28
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-22 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 172.399, 172.399, 135.963 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.735 - 2.796 |
| R-factor | 0.1883 |
| Rwork | 0.187 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tg6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.181 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.796 | 2.800 |
| Rmerge | 0.179 | |
| Rmeas | 0.188 | 0.188 |
| Rpim | 0.056 | 0.566 |
| Number of reflections | 57827 | 2896 |
| <I/σ(I)> | 15.4 | 1.27 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.1 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 294 | 0.1 M sodium acetate trihydrate pH 4.6, 4% w/v polyethylene glycol 4,000 |
