6BB4
Fab/epitope complex of mouse monoclonal antibody C5.2 targeting a phospho-tau epitope.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-20 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 161.156, 40.535, 228.064 |
Unit cell angles | 90.00, 104.07, 90.00 |
Refinement procedure
Resolution | 44.250 - 2.099 |
Rwork | 0.218 |
R-free | 0.26990 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER (1.12_2829) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.250 | 2.174 |
High resolution limit [Å] | 2.099 | 2.099 |
Rmerge | 0.069 | 0.544 |
Rmeas | 0.079 | 0.625 |
Rpim | 0.039 | 0.303 |
Number of reflections | 84763 | 8330 |
<I/σ(I)> | 12.34 | 2.51 |
Completeness [%] | 99.5 | 99.32 |
Redundancy | 4.2 | 4.2 |
CC(1/2) | 0.999 | 0.890 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 25.5% polyethylene glycol 4000, 0.17 M ammonium sulfate, 15% glycerol |