6BAB
The structure of human CamKII with bound inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.250, 67.570, 82.840 |
| Unit cell angles | 93.34, 92.93, 90.11 |
Refinement procedure
| Resolution | 54.180 - 1.910 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.825 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 54.200 |
| High resolution limit [Å] | 1.910 |
| Number of reflections | 80037 |
| <I/σ(I)> | 7.6 |
| Completeness [%] | 87.4 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | Sitting drop vapor diffusion droplets were assembled with 250 nL of 12 mg/mL CamKII, 0.6 mM inhibitor and 250 nL of reservoir solution 24% peg 3350, 0.2 M ammonium tartrate, 0.1 M arginine. |
