6BA5
Potent and Selective Antitumor Activity of a T-Cell Engaging Bispecific Antibody Targeting a Membrane-Proximal Epitope of ROR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.020, 73.900, 84.120 |
| Unit cell angles | 89.86, 71.30, 84.49 |
Refinement procedure
| Resolution | 39.530 - 1.620 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Antibody R11 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.850 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.087 | 0.497 |
| Rmeas | 0.103 | 0.595 |
| Rpim | 0.054 | 0.321 |
| Number of reflections | 160894 | 7849 |
| <I/σ(I)> | 8.5 | 2.5 |
| Completeness [%] | 96.1 | 94.6 |
| Redundancy | 3.7 | 3.3 |
| CC(1/2) | 0.993 | 0.717 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 0.2 M Li Sulfate, 20% PEG3350 |
