6B65
IMPase (AF2372) R92Q/K164E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-08-31 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 |
Unit cell lengths | 90.315, 90.315, 101.412 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.480 - 2.702 |
R-factor | 0.2006 |
Rwork | 0.198 |
R-free | 0.24670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lvb |
RMSD bond length | 0.018 |
RMSD bond angle | 1.236 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.480 |
High resolution limit [Å] | 2.700 |
Number of reflections | 24448 |
<I/σ(I)> | 2.776 |
Completeness [%] | 96.5 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 100 mM myo inositol 14% PEG 3350 200 mM potassium sulfate 100 mM magnesium chloride 100 mM Hepes 7.0 |