6B5L

Structure of PfCSP peptide 20 with human protective antibody CIS43

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	93.942, 61.668, 75.371
Unit cell angles	90.00, 106.04, 90.00

Refinement procedure

Resolution	45.140 - 2.400
R-factor	0.201
Rwork	0.199
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.826
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.142
High resolution limit [Å]	2.400
Number of reflections	15363
<I/σ(I)>	6.1
Completeness [%]	93.3
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Ammonium Sulfate, 0.1M Sodium 659 Acetate Trihydrate pH 4.6, 30% PEG MME 2000