6B2N
Crystal structure of TEM-1 beta-lactamase mutant M182N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2017-04-03 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.910, 49.660, 122.160 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 19.767 - 2.000 |
| R-factor | 0.2347 |
| Rwork | 0.231 |
| R-free | 0.28280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jwp |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.582 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 66091 | |
| <I/σ(I)> | 8.95 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291 | 0.1 M sodium phosphate dibasic/citric acid, pH 4.2, 0.1 M lithium sulfate, 20% PEG1000 |
