6B27

Crystal structure of human STAC2 Tandem SH3 Domains (296-411) in complex with a CaV1.1 II-III loop peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	47.920, 114.650, 144.678
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 1.730
R-factor	0.1804
Rwork	0.179
R-free	0.21630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.935
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	1.780
High resolution limit [Å]	1.730	3.960	1.730
Rmerge	0.058	0.023	0.493
Rmeas	0.068	0.027	0.577
Rpim	0.035	0.014	0.294
Total number of observations	301764
Number of reflections	83341	7304	6866
<I/σ(I)>	7.8
Completeness [%]	99.1	98.5	99.6
Redundancy	3.6	3.5	3.6
CC(1/2)		0.998	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	277.15	2.24M ammonium sulfate, 0.1M sodium acetate