6B0B

Crystal structure of human APOBEC3H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.28
Spacegroup name	P 31 1 2
Unit cell lengths	101.293, 101.293, 211.217
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.250 - 3.280
R-factor	0.353353535773
Rwork	0.353
R-free	0.36244
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.574
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXD
Refinement software	PHENIX (dev_2926)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	3.480
High resolution limit [Å]	3.280	3.280
Rmerge	0.210	3.200
Rpim	0.048
Number of reflections	366518	37356
<I/σ(I)>	13.4	0.7
Completeness [%]	98.3	89
Redundancy	20.4	21.1
CC(1/2)	0.999	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	NH4I, PEG3350