6AZZ
Crystal structure of Pfs25 in complex with the transmission blocking antibody 1190
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-13 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.572, 203.136, 65.486 |
Unit cell angles | 90.00, 109.89, 90.00 |
Refinement procedure
Resolution | 39.179 - 2.400 |
R-factor | 0.1921 |
Rwork | 0.191 |
R-free | 0.21690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z27 1276 Fab |
RMSD bond length | 0.002 |
RMSD bond angle | 0.544 |
Data reduction software | XDS |
Data scaling software | XPREP |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rpim | 0.068 | 0.330 |
Number of reflections | 51230 | 5894 |
<I/σ(I)> | 11.5 | 2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.085 M Tris pH 8.5, 0.17 M sodium acetate, 25.5 % (w/v) PEG 4000 |