6AZE
Crystal Structure of the BPTF PHD-bromodomain module bound to H3KC4me3 methyl lysine analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.263, 64.130, 85.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.623 - 2.451 |
R-factor | 0.1814 |
Rwork | 0.180 |
R-free | 0.21310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.469 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.5) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 2.490 |
High resolution limit [Å] | 2.450 | 6.610 | 2.450 |
Rmerge | 0.103 | 0.066 | 0.319 |
Number of reflections | 6994 | 367 | 307 |
<I/σ(I)> | 7 | ||
Completeness [%] | 85.2 | 78.9 | 76.6 |
Redundancy | 4.8 | 5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1uL complex solution (~8 mg/ml of [1:1.2 protein to peptide molar ratio] in 100 mM KCl, 10 mM HEPES KOH, pH 7.5 and 5 mM DTT) + 1 uL reservoir (12% (w/v) polyethylene glycol 6000, 5% glycerol, 100 mM KCl, and 10 mM MgCl2 buffer), equilibrated against 1mL of reservoir in Nextal hanging drop plate format. |