6AXG

Structure of RasGRP4 in complex with HRas

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 32 1 2
Unit cell lengths	114.105, 114.105, 680.872
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.210 - 3.302
R-factor	0.2295
Rwork	0.227
R-free	0.26020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4l9m
RMSD bond length	0.003
RMSD bond angle	0.776
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.210	3.370
High resolution limit [Å]	3.300	3.300
Rmerge	0.224	0.612
Number of reflections	76133
<I/σ(I)>	8.3	1.9
Completeness [%]	98.7
Redundancy	9.2
CC(1/2)	0.968	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG3350 and 300 mM sodium thiocyanate