6AUN
calcium-independent phospholipase A2 beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2015-06-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 62 |
| Unit cell lengths | 266.061, 266.061, 79.375 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.112 - 3.951 |
| R-factor | 0.2618 |
| Rwork | 0.257 |
| R-free | 0.30970 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.061 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.950 | 4.090 |
| High resolution limit [Å] | 3.950 | 3.950 |
| Rmerge | 0.101 | 1.080 |
| Rpim | 0.044 | 0.460 |
| Number of reflections | 24660 | 906 |
| <I/σ(I)> | 17 | 4.3 |
| Completeness [%] | 86.3 | 32 |
| Redundancy | 6.2 | |
| CC(1/2) | 0.700 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 5.5 | 293 | 0.1 M Bis-Tris, pH 5.5, 10% PEG3350, 0.2 M sodium/potassium salt |
