6ASO

Structure of yeast U6 snRNP with 3'-phosphate terminated U6 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	94.197, 77.390, 118.614
Unit cell angles	90.00, 105.91, 90.00

Refinement procedure

Resolution	55.630 - 2.710
R-factor	0.199
Rwork	0.195
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vsu
RMSD bond length	0.011
RMSD bond angle	1.656
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.590	2.910
High resolution limit [Å]	2.711	2.800
Rmerge	0.446
Rpim	0.058	2.180
Number of reflections	40745	4574
<I/σ(I)>	8.7	0.5
Completeness [%]	100.0	100
Redundancy	60.7	58.5
CC(1/2)	0.997	0.418

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	280 mM sodium potassium tartrate 22.5 % glycerol 17.5 % PEG 3,350 1 mM manganese chloride