6AS7

CRYSTAL STRUCTURE OF THE CATALYTIC CORE OF HUMAN DNA POLYMERASE ALPHA IN TERNARY COMPLEX WITH AN DNA-PRIMED DNA TEMPLATE AND DCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 42 21 2
Unit cell lengths	151.807, 151.807, 113.299
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.200 - 2.950
Rwork	0.259
R-free	0.30100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qcl
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.950	8.000	2.950
Rmerge	0.072	0.033	0.470
Number of reflections	28565	1555	1406
<I/σ(I)>	18.2
Completeness [%]	99.6	97.4	99.3
Redundancy	9.6	12.1	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	6MM COCL2, 2 MM TCEP, 50 MM NA ACETATE PH 4.6, 225 MM 1,6HEXADEDIOL