6AQR
SAGA DUB module Ubp8(C146A)/Sgf11/Sus1/Sgf73 bound to monoubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.755, 103.177, 112.846 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.150 - 2.100 |
| R-factor | 0.2036 |
| Rwork | 0.201 |
| R-free | 0.24880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mhs |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.855 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.920 | 46.920 | 2.160 |
| High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
| Rmerge | 0.093 | 0.052 | 0.572 |
| Rmeas | 0.103 | 0.057 | 0.630 |
| Rpim | 0.043 | 0.024 | 0.259 |
| Total number of observations | 304961 | 3983 | 24852 |
| Number of reflections | 53790 | 761 | 4370 |
| <I/σ(I)> | 13.3 | 35.9 | 3 |
| Completeness [%] | 99.2 | 94.8 | 99.7 |
| Redundancy | 5.7 | 5.2 | 5.7 |
| CC(1/2) | 0.996 | 0.995 | 0.825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M HEPES pH 7.0 17% PEG3350 0.1 M ammonium sulfate |
