6AOZ

Crystal structure of human FLASH N-terminal domain C54S/C83A (Crystal form 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	39.281, 41.086, 60.951
Unit cell angles	95.58, 105.18, 113.40

Refinement procedure

Resolution	36.721 - 2.100
R-factor	0.2175
Rwork	0.215
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ano
RMSD bond length	0.003
RMSD bond angle	0.405
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.721	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.428	0.428
Rpim	0.278
Number of reflections	18642	1888
<I/σ(I)>	18.2	3.25
Completeness [%]	97.9	97.7
Redundancy	3.9	3.4
CC(1/2)	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	4% (w/v) tacsimate pH 7.0, 11 % (w/v) PEG 3350