6AOJ

Crystal structure of Legionella pneumophila effector Ceg4 with N-terminal yeast Hog1p sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	78.970, 75.923, 47.911
Unit cell angles	90.00, 104.17, 90.00

Refinement procedure

Resolution	32.529 - 1.902
R-factor	0.164
Rwork	0.162
R-free	0.19270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6aok
RMSD bond length	0.009
RMSD bond angle	0.954
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (dev_2733)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.052	0.259
Rpim	0.031	0.149
Number of reflections	20881	992
<I/σ(I)>	39.3	4.5
Completeness [%]	97.2	92.5
Redundancy	3.9	3.8
CC(1/2)		0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.2 M sodium chloride, 0.1 M bis-Tris pH 6.5, 25% (w/v) PEG3350