6AOJ
Crystal structure of Legionella pneumophila effector Ceg4 with N-terminal yeast Hog1p sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.970, 75.923, 47.911 |
| Unit cell angles | 90.00, 104.17, 90.00 |
Refinement procedure
| Resolution | 32.529 - 1.902 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.19270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6aok |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.954 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2733) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.052 | 0.259 |
| Rpim | 0.031 | 0.149 |
| Number of reflections | 20881 | 992 |
| <I/σ(I)> | 39.3 | 4.5 |
| Completeness [%] | 97.2 | 92.5 |
| Redundancy | 3.9 | 3.8 |
| CC(1/2) | 0.961 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2 M sodium chloride, 0.1 M bis-Tris pH 6.5, 25% (w/v) PEG3350 |
