6AO0
CAT192 Fab Insertion Mutant H2/L2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-03 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5419 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.060, 86.590, 45.890 |
| Unit cell angles | 90.00, 100.57, 90.00 |
Refinement procedure
| Resolution | 31.237 - 2.350 |
| R-factor | 0.2082 |
| Rwork | 0.206 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6amj |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.483 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.237 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.332 | |
| Number of reflections | 16303 | 7335 |
| <I/σ(I)> | 2.9 | |
| Completeness [%] | 85.1 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 2M Ammonium sulfate, 0.1M sodium acetate trihydrate pH 4.6 |
