6ANK
Synaptotagmin-7, C2A- and C2B-domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.637, 75.059, 70.980 |
| Unit cell angles | 90.00, 114.51, 90.00 |
Refinement procedure
| Resolution | 44.456 - 2.254 |
| R-factor | 0.218 |
| Rwork | 0.214 |
| R-free | 0.25680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n5a 6anj |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.489 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.460 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.079 | 0.766 |
| Rpim | 0.046 | |
| Number of reflections | 25080 | |
| <I/σ(I)> | 15.4 | 1.29 |
| Completeness [%] | 91.6 | 56.3 |
| Redundancy | 3.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20% PEG 3350, 0.2 M lithium sulfate, 0.1 M Bis-Tris, 0.1 M calcium chloride, 0.125 M KCl, 20% ethylene glycol |
