6AL8
Crystal structure HpiC1 Y101F/F138S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.990, 47.951, 174.765 |
| Unit cell angles | 90.00, 97.05, 90.00 |
Refinement procedure
| Resolution | 47.502 - 1.641 |
| R-factor | 0.2248 |
| Rwork | 0.223 |
| R-free | 0.25380 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.815 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.502 |
| High resolution limit [Å] | 1.640 |
| Number of reflections | 122677 |
| <I/σ(I)> | 13.42 |
| Completeness [%] | 97.7 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% MEPEG 5000, 100 mM BisTris pH 6.5, 5% ethylene glycol |
