6AKF
Crystal structure of mouse claudin-3 P134A mutant in complex with C-terminal fragment of Clostridium perfringens enterotoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.540, 127.450, 165.700 |
| Unit cell angles | 90.00, 104.53, 90.00 |
Refinement procedure
| Resolution | 46.660 - 3.900 |
| R-factor | 0.2758 |
| Rwork | 0.274 |
| R-free | 0.31200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ake |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.593 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.660 | 4.040 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.091 | 1.032 |
| Rmeas | 0.109 | 1.244 |
| Rpim | 0.059 | 0.682 |
| Number of reflections | 25301 | 2529 |
| <I/σ(I)> | 8.17 | 1.32 |
| Completeness [%] | 98.5 | 99.49 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.999 | 0.575 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293.15 | HEPES, Sodium acetate, Magnesium nitrate, PEG 3350 |
